reporter gene assay, using the method of Miller, we found the activity of the b-galactosidase.
substrate was added to samples containing other compounds to see if these interfere with the activity of the enzyme, the product that gives this reaction with ONPG is yellow and is measured in a spectrophotometer. We
aliquot tubes last week and we add Buffer Z. SDS and chloroform to put that then to put ONPG, this substance can come, adding that, the b-galactosidase to catalyze the reaction starts and when the product (the yellow) it stops with Na2CO3 and placed in cold. (We duplicate samples and every 30 seconds to add ONPG tubes). We note
initial time and final time of the reaction. We take the cell density of the surplus ml tubes at an absorbance at 570 nm. Absorbance for Miller units at 420 nm. Make a table that includes all values \u200b\u200band also a chart appears in Miller units versus time.
substrate was added to samples containing other compounds to see if these interfere with the activity of the enzyme, the product that gives this reaction with ONPG is yellow and is measured in a spectrophotometer. We
aliquot tubes last week and we add Buffer Z. SDS and chloroform to put that then to put ONPG, this substance can come, adding that, the b-galactosidase to catalyze the reaction starts and when the product (the yellow) it stops with Na2CO3 and placed in cold. (We duplicate samples and every 30 seconds to add ONPG tubes). We note
initial time and final time of the reaction. We take the cell density of the surplus ml tubes at an absorbance at 570 nm. Absorbance for Miller units at 420 nm. Make a table that includes all values \u200b\u200band also a chart appears in Miller units versus time.
While we thought we had worked well and the absorbance were quite successful in the time to make the graphics got very poorly and a straight illogical.
Figure if we had done well would have been something like
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