Population And Genetics Ap Lab

Second week (22/05/08)

sow bacteria E. Coli with a plasmid into, to massively multiply their numbers and so have many plasmids can then use for cloning.

To achieve this we had to do the following:

prepare the LB growth medium (NaCl 1%, yeast extract 0.5% Peptone 1%), to weigh the components we use an analytical balance and then with a test tube, add 100 ml of distilled water. Labeled and sent to be sterilized (30 minutes) with about three Erlenmeyer flasks. Put the medium in the Erlenmeyer flasks and add 10 ul of 100mg/ml ampicillin (1000x) with a micropipette.

Then sow the bacterium E. Coli (strain DHSα) with plasmids (YEP-357). We put the Erlenmeyer flasks in an incubator at 37 ° C (shaker).

We used this strain of E. coli because we want to do is to differentiate the bacteria with the plasmid of which do not have and this is achieved with this strain, which is sensitive to ampicillin. Plasmid achieves the bacteria that has built not die and do not have it together. We use this plasmid
because it can replicate in yeast and bacteria and also has a reporter gene (β-galactose).

* The image is plasmid-357 Yep.

Blood Banks Pay Cash Miami

first week (05/15/2008)

Upon arrival at the laboratory after an exhaustive search by the university, we expect the researcher Susana Correa García. As she explained to us what it is about the internship, some theoretical data and what we had planned to be added also to the assistant Sabrina Cardillo talk with which we work most time.
Then at 10 am we left to attend an informative lecture was provided by the UBA. This consisted of two videos and explained and appointed safety standards and that one should work and what to do in emergencies.
After the talk returned to the laboratory and followed us alone Sabrina instructed on theoretical content, explained the deeper themes and techniques that we would use appointed during the internship (PCR), showed us how some teams and some materials. We also sent information on the subject via the Internet.

Some topics we discussed:
Bacteria: E.
Coli Gene: Saccharomyces cerevisiae UGA4 Technical
cloned: PCR

Ivan and Victoria

C-section Incision Dressing

Chemistry Project Human Genome ORT

Research in Biochemistry and Molecular Proteomics 2008

For the fourth year students in the last year of the specialty chemical ORT Technical School (Argentina) made their final projects at various universities and research centers with recognized professionals. The working methodology avoids the didactic transposition allowing students to make their final activity where knowledge is being generated.

The relationship between the average level toward degree is sponsored by the CONICET (National Council for Scientific and Technical Research.

research projects for the current year are:

1. Expression Evaluation adhesion molecule epithelial cadherin in human normal and tumor tissues.
Investigator: Dr. Monica Vazquez-Levin.
Institute: Institute of Molecular Biology and Medicine (IBYME - CONICET).
Students: Yael Dobzewicz Y Gala Szapiro.
Link: http://www.proyecto-6q.blogspot.com/

2. Action hexachlorobenzene (HCB) on uroporphyrinogen a human hepatocyte cell line. Mechanism of action.
Researcher: Dr. Maria del Carmen Ríos de Molina.
Institute: Biological Chemistry Department, Faculty of Natural Sciences.
Students: Lucas Toiw and Uriel Frid.
Link: http://www.proyectofrid-toiw08.blogspot.com/

3. Experimental models of metabolic diseases: porphyria and Metabolic Syndrome. Proteomics and Metabolomics of these disturbances.
Investigator: Dr. Marta White Mazzetti.
Institute: Biological Chemistry Department, Faculty of Natural Sciences (UBA).
Students: Maria Duperron and Mauro Elencwajg.
Link: http://proyectofinal6q.blogspot.com/

4. Hemodynamic Factors not related to the genesis and evolution of proteinuria in progressive renal disease.
Investigator: Dr. Elsa Zotta.
Institute: physiopathology Laboratory, Department of Physiology, Faculty of Medicine, UBA.