Growth prepare three media: LB
Middle (for growing bacteria):
· Extract 0.5% yeast
·
· NaCl 1% 1% Peptone Agar 2%
·
YPD medium (rich medium to grow yeast)
• And (yeast extract) 1%
· P (peptone) 2%
· D (dextrose / glucose) 2% 2% Agar
·
Middle YNB-DO (URA minimal medium (-) to grow yeast)
· YNB (yeast nitrogen base) 0.67%
· Glucose 2% Drop out
· URA (-) 0.19% Agar 2.5%
·
All agar media were added because we do plates.
Autoclaving the media along with tips and Erlenmeyer flasks to sterilize.
prepare 15 plates with media, 5 plates for each and labeled.
prepare a buffer that was needed in the laboratory, Z buffer used, inter alia, for the testing of B-gal two weeks ago. We weighed
components as we did with media, but without autoclaving. Susana
explained as primers are constructed and gave us a problem to do.
Middle (for growing bacteria):
· Extract 0.5% yeast
·
· NaCl 1% 1% Peptone Agar 2%
·
YPD medium (rich medium to grow yeast)
• And (yeast extract) 1%
· P (peptone) 2%
· D (dextrose / glucose) 2% 2% Agar
·
Middle YNB-DO (URA minimal medium (-) to grow yeast)
· YNB (yeast nitrogen base) 0.67%
· Glucose 2% Drop out
· URA (-) 0.19% Agar 2.5%
·
All agar media were added because we do plates.
Autoclaving the media along with tips and Erlenmeyer flasks to sterilize.
prepare 15 plates with media, 5 plates for each and labeled.
prepare a buffer that was needed in the laboratory, Z buffer used, inter alia, for the testing of B-gal two weeks ago. We weighed
components as we did with media, but without autoclaving. Susana
explained as primers are constructed and gave us a problem to do.
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