We PCR to amplify the insert (800 bp promoter UGA3), and cut with restruccion enzymes (EcoRI and Bami .) When we add vector tube and let phosphatase in oven at 37 ยบ C for an hour and a half to be desfosfate well. After three buffers purify the vector and insert.
prepare an agarose gel where we put the inserts and let it run.
prepare an agarose gel where we put the inserts and let it run.
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