note the results of the previous week. We used the plasmid to purify one of the weeks and cut with a restriction enzyme ( HindIII .) We did it twice.
This is called digestion of a plasmid . It uses a buffer specific enzymes, the plasmid and filled with water. Then placed at 37 ° C during the period of one hour and a half.
In agarose gel 8% m / v ran the undigested plasmid at different concentrations and the two plasmids also digested in different concentrations.
The image shown in the first two wells the undigested plasmids (the two bands observed belong to the two ways in which this CCC (predominant) and OC (one of the chains broke leaving plasmid in a relaxed state). The following wells show the once digested plasmid at different concentrations, where you see a band that belongs to the state of linear plasmid (cut the two strings). At the end is the marker and use it to measure the amount of base pairs so make sure everything ran correctly.
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