We found what we were wrong last time, and after going back to Excel all we realized that the mistake he had was some brackets in the equations to get Miller units. With the corrected data, we figure in a special program and found that it was actually correct as is the experience.
Sabrina gave us a method protocol Chip (chromatin immunoprecitation) in English, to familiarize with the vocabulary and be able to deduce and understand what each step was and what was required.
This technique consists basically of:
· Fix or leave everything static (covalently joining if any, binding to the protein) using formaldehyde
§ comparing sonicated to break DNA into smaller fragments
· is added to a protein that helps clean up the proteins that are more
· is puts an antibody that attaches to the protein that interests me
§ comparing add a ball of protein that is also attached to the antibody and this precipitated PCR
· is made and analyzes the content.
This technique consists basically of:
· Fix or leave everything static (covalently joining if any, binding to the protein) using formaldehyde
§ comparing sonicated to break DNA into smaller fragments
· is added to a protein that helps clean up the proteins that are more
· is puts an antibody that attaches to the protein that interests me
§ comparing add a ball of protein that is also attached to the antibody and this precipitated PCR
· is made and analyzes the content.
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