Saturday, June 28, 2008

Ativan How Long Before Drinking

seventh week (26/06/08)

induction.
Day 1: take a colony of yeast containing a plasmid (YEP -357) and placed on minimal medium, leave it overnight.
Day 2: it is a dilution of the medium and leaving it overnight.
Day 3: the medium is divided into three tubes are centrifuged pellet staying with and adding back the minimal media but cool (one of the three tubes were added Leucine (un aminoácido )). Después de 30 minutos al tubo de los aminoácidos y a otro se le agrega GABA .
Se toma una muestra a tiempo 0 de los tres tubos y otras a los 120 minutos.
El objetivo es saber en que condiciones el gen se induce mejor, si afecta la presencia de aminoácidos en la inducción y como se ven estos cambios según la variación del tiempo.

Sabrina también nos explicó como hacer para saber si una proteína interactúa con el gen UGA 4. para eso se usa formaldehido que une covalentemente a la proteína con el gen. Después un antibody and agarose ball bind with the protein. This is precipitated using the centrifuge.
After that you can have 2 possibilities: that the protein sticks to UGA 4 and rush with this, or stick to something else and is the UGA gene 4 in the supernatant . If UGA is
4 in the supernatant means that the protein does not interact with it.
was verified by PCR

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