Population And Genetics Ap Lab

Second week (22/05/08)

sow bacteria E. Coli with a plasmid into, to massively multiply their numbers and so have many plasmids can then use for cloning.

To achieve this we had to do the following:

prepare the LB growth medium (NaCl 1%, yeast extract 0.5% Peptone 1%), to weigh the components we use an analytical balance and then with a test tube, add 100 ml of distilled water. Labeled and sent to be sterilized (30 minutes) with about three Erlenmeyer flasks. Put the medium in the Erlenmeyer flasks and add 10 ul of 100mg/ml ampicillin (1000x) with a micropipette.

Then sow the bacterium E. Coli (strain DHSα) with plasmids (YEP-357). We put the Erlenmeyer flasks in an incubator at 37 ° C (shaker).

We used this strain of E. coli because we want to do is to differentiate the bacteria with the plasmid of which do not have and this is achieved with this strain, which is sensitive to ampicillin. Plasmid achieves the bacteria that has built not die and do not have it together. We use this plasmid
because it can replicate in yeast and bacteria and also has a reporter gene (β-galactose).

* The image is plasmid-357 Yep.